A detailed mechanism of the oxidative half-reaction ofd-amino acid oxidase: another route for flavin oxidation
نویسندگان
چکیده
منابع مشابه
Reaction mechanism of flavin dehydrogenation by D-amino acid oxidase
Introduction Since its discovery, D-amino acid oxidase (EC 1.4.3.3, DAAO) has received much attention both from the physiological and biochemical points ofview. The groups of Massey (USA), Curti (1taly), and Yagi (Japan) were mainly involved in its study over the year (1). In spite of these efforts the basic molecular mechanism underlying the reductive half-reaction, i.e. the dehydrogenation st...
متن کاملpH and kinetic isotope effects on the oxidative half-reaction of D-amino-acid oxidase.
D-Amino-acid oxidase catalyzes the oxidation of D-amino acids to imino acids. In the oxidative half-reaction, oxygen reacts with the reduced enzyme-imino acid complex to reoxidize the bound FAD. This is then followed by dissociation of the imino acid. The effects of pH and D2O on the kinetics of the oxidative half-reaction of D-amino-acid oxidase have been determined with glycine, D-alanine, an...
متن کاملInterpretation of the Amino Acid Oxidase pH Dependence of Flavin Reduction in the L - Reaction
The pH profiles of the apparent biomolecular rate constants for the reaction f free EO with [2-'H]and [22H]phenylalanine (evaluated as (kr)H and (kr)H*, respectively, from rapid kinetic measurements and as the equivalent steady state coefficients ( & l ) ~ and (+I-')"*) show that k , = k', k2 >> k-' << k2* and that the ionization controlled by K', (= kL,/k,) is at virtual equilibrium. The expre...
متن کاملProton release during the reductive half-reaction of D-amino acid oxidase.
Changes in the net protonation of D-amino acid oxidase during binding of competitive inhibitors and during reduction by amino acids have been monitored using phenol red as a pH indicator. At pH 8.0, no uptake or release of protons from solution occurs upon binding the inhibitors benzoate, anthranilate, picolinate, or L-leucine. The Kd values for both picolinate and anthranilate were determined ...
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It has been known since the studies of Neubauer in 1909 and of Knoop in 19107"' that the mammalian organism is able to degrade a-amino acids to a-keto acids. Knoop advanced the hypothesis that this degradation involved an oxidative deamination.' According to this hypothesis, the imino derivative of the amino acid is first formed in an oxidative step and, in turn, the imino compound yields the c...
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ژورنال
عنوان ژورنال: Organic & Biomolecular Chemistry
سال: 2019
ISSN: 1477-0520,1477-0539
DOI: 10.1039/c9ob00975b